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Plate and inoculate BL21+pGFP(LVA)
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Quantitate the Constitutive Expression
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Quantitate the Constitutive Expression
Extract GFP(LVA) insert
From E coli BL21+pGFP(LVA) or by creating
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Plate and inoculate BL21+pGFP(LVA)
Transform with pGFP(LVA)
Miniprep to extract plasmid
Restriction digest of pGFP(LVA) with HindIII
Separate digested GFP(LVA) insert with QIAEX agarose gel extraction kit
Second Attempt: Restriction Digest of pGFP(LVA)
Second Attempt: Separate digested GFP(LVA) insert with QIAEX agarose gel extraction kit
Ron Weiss
Wed Feb 10 15:48:08 EST 1999
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