Plate and inoculate BL21+pGFP(LVA)

 

Materials:

Methods:

1. Thaw E coli on ice
2. Fill three 1.5ml eppendorf tubes with of LB Amp liquid each for serial dilutions
3. perform serial dilutions of thawed stab cultures: (assume initial density):
   Aliquot from stab into 1.5ml eppendorf tube labeled `` dilution'', vortex.
   Aliquot from `` dilution'' tube into `` dilution'' tube (approx density: ), vortex.
   Aliquot from `` dilution'' tube into `` dilution'' tube (approx density: ), vortex.
4. Plate (and label) each of the following unto a seperate LB Amp plate:
   

   

5. grow plates overnight in .
6. Pick strongly glowing colonies of BL21+pGFP(LVA) from plates and inoculate into LB Amp culture flasks. Grow overnight .

Results: ( 1/22-1/24/99)

Got only one colony from all plates (on dilution). Therefore, performed transformation of   with pGFP(LVA) to get sufficient amounts of the needed plasmid.