Gel Purify Ligation of pBR322 + GFP(LVA)

 

Materials:

Methods:

1. Pour a 1.0% agarose gel according to protocol in appendix D.1.
2. set up DNA ladder: 11    , 5   of 123 bp DNA ladder, 4   gel loading buffer (20   total)
3. set up 3 sample tubes: 20   of each ligation separate tubes, add 5   of gel loading buffer (total: 25  )
4. load the wells in the following order: G1, ladder, G2, G3.
5. run the gel for 1.5 hrs @70V, or until fast dye has reached 2/3 of the gel.
6. separate and excise the pBR322+GFP(LVA) ligated plasmid according to protocol in appendix F.1.
7. use the DNA for electroporation.

Results: ( 2/10/99)
Started running the gel backward, but checked after 5 min, and corrected...