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Create additional pBR322 vector

Inoculate and Grow
Transform with pBR322, plate, and grow
Inoculate into LB Amp liquid and grow
Miniprep to extract plasmid
Restriction digest of pBR322 with EcoRI
Analytical Gel to Check pBR322 digest
Second Attempt: Restriction Digest of pBR322
Second Attempt: Separate digested pBR322 insert with QIAEX agarose gel extraction kit

Ron Weiss
Wed Feb 10 15:48:08 EST 1999