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- (a) Analytical gel electrophoresis on maxiprep with
both pBR322 and pGFP(LVA), with a 1kb ladder. pBR322 shows nicely in
the right two lanes, while pGFP(LVA) is absent. (b) Analytical
gel electrophoresis on maxiprep for pGFP(LVA). This time, pGFP(LVA)
is quite noticeable in the right two lanes, next to a 123bp ladder.
The maxiprep debug samples are not noticeable.
- (a) Analytical gel electrophoresis (1.5 hours,
) on restriction digest samples. Lanes have: 1 = 1kb ladder, 2 =
pBR322 cut with EcoRI sample, 3 = pGFP(LVA) sample
cut with
HindIII, 4 = pGFP(LVA) sample
cut with HindIII, 5 = 1kb ladder.
(b) 1 kb ladder, not shown to scale with other figure.
- Seperation gel electrophoresis (1.5 hours,
)
on restriction digest samples, with two 2-well combs. Lanes have: top
left = nothing, top right = pGFP(LVA) sample
cut with HindIII,
mid left = pBR322 cut with EcoRI sample, mid right = pGFP(LVA) sample
cut with HindIII)
- (a) Gel before restaining, showing only ladders and
pGFP(LVA) without GFP(LVA) insert. (b) Gel after staining, now
also showing pBR322 vector and GFP(LVA) insert. [Note: figs not to
scale]
-
genotype
Ron Weiss
Wed Feb 10 15:48:08 EST 1999