Agarose Gel Electrophoresis

 

Some of the protocol from Winfrey.

Materials:

Methods:

1. Before running the gel, add loading dye to each sample (to 1X concentration), and use   for rest. Examples:

   

2. For a well of a DNA ladder: dna ladder, of loading buffer,  .
3. Pour 1X TAE into the horizontal electrophoresis chamber (p. 32). First pour enough to cover middle of gel apparatus.
4. Place gel in apparatus, and add 1X TAE to completely cover wells.
5. Pipet EtBr (   per 10 ml of TAE, using EtBR) into gel apparatus chamber close to red. For small cast, use about 40   EtBr.
6. Briefly microfuge samples.
7. Pipet samples and ladders into wells. Turn power on. Voltage depends on gel properties (e.g. for agarose gel, sometimes 85V for small cast, 106V for large cast).