LB Amp plates

 

Materials:

Methods:

1. Make ``LB Agarose'' liquid. Find Agarose in cabinet, and add to LB liquid in bottle (1 liter LB, 40 g agarose, or 15 g/liter agar technical). Shake hard to dissolve the agarose in LB.
2. Make sure caps are loose. Autoclave for 30 min . Before autoclaving, make sure water level is sufficiently high (below flat carving, above round carving). Autoclaving will take approx 1.5 hours.
3. Thaw filtered ampiclin (100 mg/ml).
4. Cool bottle to (with a water bath).
5. Aliquot Amp to achieve 100  /ml final concentration. Shake well.
6. Pour 30-35 ml into each plate.
7. Stack on top of each other to avoid condensation. Let cool until solid .
8. Optional: incubate plates with top partially off for 1 hour to get rid of condensation.