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put pGBM7 into  

 

Methods:

  1. streak, pick, and culture tex2html_wrap_inline4459   in (??? medium)
  2. mini-prep
  3. electroporate pGBM7 into tex2html_wrap_inline3066  
  4. culture in rescue medium (SOB/SOC?)
  5. innoculate a culture into LB Amp (medium???)
  6. streak on LB Amp
  7. look for glowing colonies
  8. innoculate some glowing colonies into LB Amp
  9. mini-prep some resultant strains, verify presence of BOTH plasmids


Ron Weiss
Wed Feb 10 15:48:08 EST 1999