next up previous contents
Next: Run stained cells through Up: Quantitate expression levels of Previous: Obtain calibration curve with

Stain cells to enable distinguishing cells from debris

 

tabular313

Materials:

tabular1884

Methods:

  1. Retrieve cells while still in log growth...
  2. Stain with SYTO 59 (red) to distinguish all bacteria from debris
  3. wash
  4. Maybe: stain with SYTOX Green to distinguish live/dead bacteria


tabular313



Ron Weiss
Wed Feb 10 15:48:08 EST 1999