Experiment I: Constant Signalling
•Figure shows fluorescence response of receiver (pRCV-3)
–Several cultures grown seperately overnight @37°C
–Cultures mixed in 5 different ways and incubated in FL600 @37°C
–Fluorescence readings taken every 5 minutes for 2 hours
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Text Box: positive control
positive control
Text Box: 10X VAI extract
10X VAI extract
Text Box: direct signalling
direct signalling
negative controls