Jerome S. Rovner.
Mechanisms Controlling Copulatory Behavior in Wolf Spiders (Araneae: Lycosidae).
Psyche 78:150-165, 1971.
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MECHANISMS CONTROLLING COPULATORY
BEHAVIOR IN WOLF SPIDERS
(ARANEAE : LYCOSIDAE)
BY JEROME S. ROVNER
Department of Zoology
Ohio University
Athens, Ohio 45701
Male spideis use their palps for picking up, storing, and, finally, transferring seminal fluid to the female's copulatory apparatus. In previous papers (Rovner, in press) I described inter-generic differ- ences in palpal insertion patterns and examined temporal variation in the duration of insertion during mating in the lycosid spiders Lycosa rabida and Schizocosa saltafrix. Using the former species and taking a different perspective in the present study, I sought to determine some of the mechanisms involved in regulating the sequence of events accompanying each ~al~al insertion, as well as the role of the palps in the orientation of the male throughout mating. Experimental modification of one or the other partner was followed by the male's performance of behaviors which did not occur during normal copula- tions: courtship, disorientation, tying down the female, and "pseudo- insertions". Such data led to hypotheses concerned with the control of various elements of copulatory behavior. During mating, lycosid spiders maintain a position (Position I1 of Gerhardt, 1924) in which the male's sternum is above the female's carapace and the partners face in opposite directions (Fig. I ). Each insertion involves the male's leaning down on one side of the female and scraping one palp (the one closest to that side of the female) against her epigynum. The male's right palp serves the female's right copulatory pore; his left palp, her left pore. One or more scrapes of the palp result in engagement of the em- bolus in the copulatory pore. The latter is accompanied by hema- todochal expansion, which forces the embolus into the duct leading to the seminal receptacle. Ejaculation of seminal fluid through the embolus is presumed to occur at maximum expansion of the hema- todocha (Gering, 1953). Subsequent collapse of the hematodocha is followed by disengagement of the embolus and lifting of the palp away from the epigynum, (For details of palpal function during copulation, see Gering, 1953. The hydrostatic system involved in hematodochal expansion, as well as in locomotion, has been studied recently by Wilson, 1970.)
'This study was supported in part by Ohio University Research Grant 244. Manuscript received by the editor, September 27, 1971. 150
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19711 Rovner - Wolf Spiders 151
Fig. 3.
Male and female Lyc0.w rufiiita sn i-op~d'~. The male is above, facing the camera, and has just initiated :i psewiu-insertion with hi! left P~P.
After about half of the palpa! insertions, male L. rabida moisten the palp just used by drawing it between the chelicerae. In such cases, they then usually moisten the opposite palp. Although a bout of ~alpal moistening often involves a rapid alternation of the palps, it always is initiated in the palp which has just been used in an at- tempted or completed insertion (Rovner, in press). In about half of the insertion sequences the male, rather than moisten the palps, either remains inactive for several seconds or immediately crosses over to the female's opposite side.
Whether or not palpal moistening occurs, male L. rahida then shift over to the female's opposite side; i.e., palpal alternation is the insertion pattern in this species (Montgomery, 1903 ) . While moving from one side to the other, the male usually taps his palps against the anterior dorsal surface of the female's abdomen. At this time the female performs the only behavior shown by her during most of the copulation - abdominal swiveling. Her abdomen rotates about its longitudinal axis during each crossing by the male, thereby bring- ing the epigynurn within reach of the male's palp. The above palpal insertion sequence, which is repeated an average of about sixty times by each palp during copulation in L. rabtda, is
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Psyche [September
r-b LEAN - SWIVEL ABDOMEN
SCRAPE PALP
4
ENGAGE EMBOLUS
V)
o HEMATODOCHAL
a
Q-
0
^
0
HEMATODOCHAL
EXPANSION
COLLAPSE
L- - REMOVE EMBOLUS
1
(/)
1 -
MOISTEN SAME PALP
I MOISTEN BOTH PALPS
Fig. 2.
Sequence of events associated with each palpal insertion during copulation in Lyrosa rabida.
summarized in Fig. 2. Some of the mechanisms associated with these events were suggested by the findings of the present study. Specific questions that I was asking were these:
(I ) Given that the palps
serve for transfer of sperm, how essential is sensory information from the palps to the maintenance of the male's copulatory state? (2) Given that the palps may be aiding in a sensory capacity to locate a target, the female's epigynal openings, is information from the palps important for the male's orientation on the female? (3) Does the pattern of right-left alternation persist after unilateral mod- ification of the male, or will the spider learn to favor the functioning palp? (4) What stimulus determines which palp is moistened first after each insertion? (5) What action by the male elicits abdominal swiveling in the female?
METHODS
Over one hundred individuals of L. rfibida were collected as im- mature instars during June,
1968, in a field near Athens, Ohio,
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19711 Rowner - Wolf Spiders 153
U. S. A. Each was housed separately in a glass jar prior to pairing in the observation arena. Mealworms (Tenebrio sp.) served as food ; and cotton-stoppered, water-filled vials provided moisture. Observations on copulatory behavior were made at temperatures of 24-26OC. A manually activated Esterline Angus event recorder, at a chart speed of 15.3 cm/min, was used to record some of the data. Protocol was whispered into the microphone of a tape recorder. Recording instruments were placed on a separate table to reduce possible effects of machine noise.
This study involved fifty pairings of virgin, adult spiders. (Al- though housed individually until that time that the mating partners were placed into the arena, the spiders will be referred to in terms of this eventual pairing.)
Ten pairs of spiders were not modified
experimentally; and their behavior represented that of normal in- dividuals. The remaining forty pairs were divided into the following eight groups, each consisting of five pairs: (I) Males losing both
palps prior to the final molt; (2) Males losing both palps after the final molt; (3) Males with both palps fixed dorsally; (4) Males losing one palp prior to the final molt; (5) Males losing one palp after the final molt; (6) Males with one palp fixed dorsally; (7) Females with both copulatory pores sealed; (8) Females with one copulatory pore sealed.
Palp removal was accomplished by autotomy. During carbon dioxide-induced anesthesia, the male's palp was attached to the sub- stratum. After the male's recovery I prodded him with an artist brush and forced him to pull away from the point of attachment, which resulted in palpal autotomy at the trochantero-femoral joint. This was repeated for males undergoing loss of both palps. When
autotomy involved penultimate males, a "stump" or a complete but vestigial palp was present after the final molt. In the latter case
the tarsus lacked a genital bulb,
Fixation of a palp above the cephalothorax involved positioning the palp into' a drop of melted paraffin placed on the adjacent region of the carapace of the anesthetized spider. Paraffin was also used to cover the copulatory pores of anesthetized females. All operations were performed under a dissecting microscope. In
each experimental group of five pairs which involved unilateral modification, three individuals were treated on one side (e.g., right palp) and two on the other side (e.g., left palp). RESULTS
A variable period of time after mounting the female, many of the males of the experimental pairs performed behaviors which were not
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154 Psyche
September
Fig. 3.
Male Lycosa ro&ilia disoriented I#O* from the normal position while above the female in roNa.
observed during copulation in the normal pairs in this or previous studies of lycosid mating behavior.
Males unable to use one or both palps (due to modification of the males) performed courtship display at various times while mounted on the female. Those males possessing one usable palp often followed each successful insertion with a brief period of courtship that was initiated after the shift to the non-functional side and the subsequent adoption of a media! position. Thus the male alternated copulation with courtship. In some cases, courtship was initiated soon offer mount; e.g., one male began to display after having suc- cessfully inserted the functional palp only three times. Males possessing one usable palp always shifted either to a medial position or completely over to the non-functional side after each successful insertion. However, "when visiting the non-functional side, these males typically did not lean ventrad as steeply as did normal males. After resting momentarily, the experimental males either shifted back immediately to the functional side or initiated a bout of courtship display. The average duration of insertion of the func- tional palp in these males ranged from 7.1 sec in one male to 15.8 in another.
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19711 hntr - Wolf Spiders 155
Fig. 4. Palplesa male Lycosa rabida disoriented from the normal position and uskg his black legs I to perform elements of courtship display while above the female ;PI copula.
Disorientation was shown by most of the males which lacked the use of both palps (due to modification of the males). In this be- havior the male pivoted on the female's carapace in a clockwise or counter-clockwise direction and temporarily adopted a position other than the normal one. The most typical and most prolonged abnormal position was that In which the male was 180O out of proper align- ment, i.e., facing in the same direction as the female (Fig, 3). The next most common abnormal position was one of go0 mis-alignment, i.e., the male's longitudinal axis perpendicular to that of the female. As "copulation" progressed, the male adopted positions even further removed from the normal one by locating himself above the basal segments of the female's legs on one side (Fig. 4). While in the various abnormal positions, the male spent much of his time in courtship display.
Whether in the normal position or disoriented 180å¡ males lack- ing the use of both palps (due to modification of the males) showed bouts of rapid, oscillatory movements, in which the male either shifted from side to side or slid forward and back while above the female's carapace. Such bouts of activity were variable in duration, on the average lasting 6,s sec (N = 140). During these excited
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156 Psyche [September
movements, the male scraped his chelicerae against the female's cara- pace (or, rarely, abdomen) and alternately spread and closed the chelicerae as they slid over the female's surface. Some of these bouts terminated with the male's use of his chelicerae to grasp the rim of the female's carapace or a basal segment of one of her appendages and to lift that end of her body dorsad. Synchronous erection of his leg spines accompanied each tug by the male. The female did not respond to the male's tugs, even though: ( I ) males disoriented 180å tugged at structures near the female's face; and (2) puncture of a joint membrane and loss of a drop of hemolymph occurred in a few females due to the male's vigorous cheliceral grasping. After one or more tugs, the male became inactive but maintained his hold on the female with his chelicerae. The pattern of behavior in males lacking the use of both h alps was that of an alternation of inactive periods (with the male located medially or leaning slightly to one side of the female) with periods involving one or more of the above- described behaviors. Abdominal vibrations continued during the male's inactive periods.
Half of the males with both palps unavailable and one-fifth of the males with one palp unavailable occasionally performed "tying down". In this behavior the male pivoted on the female's carapace in a clock- wise or counter-clockwise direction while laying down a barely visible silk line over the legs and abdomen of the female. The silk on the legs usually contacted the patellar and tibia1 segments; less fre- quently, the femora. At a few points in the crude circle, the silk was attached to the substratum. During tying down the male typ- ically moved through a complete 360å and resumed the normal posi- tion. Less often the male momentarily stopped at 180å and then returned to the normal position by either continuing to 360å or re- versing direction and covering the same ground again. Even though the male stopped briefly at 180å (or, rarely, go0 or 270å¡) he did not initiate any other behavior at these points, but soon resumed tying down in a return to the normal position. Thus, tying down was distinct from the behavior described above as disorientation, in that the latter: (I) did not involve release of silk; (2) involved pivoting through arcs of 180å or less; and (3) usually was accom- panied by other behaviors while the male was in an abnormal posi- tion. Tying clown was shown in pairs in which the female was inactive throughout the copulation (except for abdominal swiveling) ; i.e., tying down typically was neither preceded nor followed by activ- ity in the female. When resuming locomotion at the end of the
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197 11 Rovner -, Wolf Spiders 157
mating, females which had undergone one to several bouts of tying down were only slightly hampered by the scant threads on their legs and readily freed themselves.
Most of the normal males copulating with females that had one or both copulatory pores sealed performed an atypical behavior which I have termed a "pseudo-insertion". This occurred when one of the palpal scrapes (insertion attempts) on an unavailable side resulted in complete expansion of the hematodocha, even though the palp was no longer in contact with the female's body. Synchronously with the hematodochal expansion, the palp was lifted dorsad, sometimes to a relatively high position (with the palpal femur about 45O above the horizontal plane). Leg spine erection accompanied hematodochal expansion, as in normal insertions.
The palp was lowered ventsad
(and the leg spines dropped) during the subsequent hematodochal collapse. Pseudo-insertion duration averaged 15.5 sec (N = 20). Pseudo-insertions began to occur during a copulation after the male had made a number of visits to an unavailable side of the female. Dur- ing each visit males made from one to ten (usually from two to five) attempts to insert. A pseudo-insertion usually occurred after five or fewer attempts; in some cases later in copulation, the first attempt during a visit to one side resulted in a pseudo-insertion. In many cases if the hematodocha began expansion after partial engagement of the embolus in the paraffin seal, expansion would continue to com- pletion after the palp slipped away from the epigynum. In other instances the palpal tarsus would swing down to the base of the female's leg IV and, meeting resistance there, give rise to a pseudo- insertion. The number of pseudo-insertions performed during such pairings ranged from one to seventeen.
Two males lacking one palp and having difficulty inserting the available palp showed pseudo-insertions, as well as courtship and tying down. Males having one or both palps fixed dorsad did not perform pseudo-insertions with their treated palps. A behavior probably related to the mechanism underlying pseudo- insertions was also seen in the males which were paired with females having one or both copulatory pores sealed. It occurred while the male was resting on one side of the female after a series of insertion attempts. (Many of these attempts involved the male's leaning steeply and scraping his palp much further posterior than the female's epigynum, sometimes thereby contacting her spinnei-ets.) During this resting period, while the male held his palp near his face, slow and weak pulsations were evident in the genital bulb. During this "throb- bing" the hematodocha was not visible. Partial, synchronous leg spine erections accompanied the genital bulb pulsations.
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158 Psyche [September
Table I.
Behaviors shown during copulation by males of treated and untreated pairs of Lycosa mbida.
(The first figure represents the number of males showing that behavior; the next figure, the number of males tested.) Both $ palps
lacking
Both 8 palps
fixed dorsad
One 3 palp
lacking
One 8 palp
fixed dorsad
Both 9
pores sealed
One 9
pore sealed
8 and 9
untreated
Courtship
7/10
5/5
4/10
4/5
1/5
215
0/10
Disorientation
6/10
4,"s
1/10
1/5
o/ 5
o/ 5
0/10
Tying
down
3/10
4/5
2/10
1/5
0/5
o/ 5
0/10
Pseudo-
insertion
-
o/ 5
2/10
o/ 5
4/ 5
41 5
0/10
Many of the above results are summarized in Table I. Data for
males treated prior to the final molt are grouped with those for males treated similarly after the final molt. There did not seem to be a difference in performance between the pre- and the po-it-molt-treated males. Further reduction of the data is provided in Table 11, in which experimental ~airings are reduced to three classes of treatment. Details of palpal moistening behavior were studied in the various experimental pairs. Males entirely lacking one palp moistened the available palp only after using it in an insertion or an insertion attempt. They did not perform moistening of the available palp after visiting the palpless side. Some males of this experimental category possessed a vestigial palp (complete but smaller and lacking a genital bulb). Insertion attempts with the latter were accom- panied by synchronous leg spine erections, but were not followed by palpal moistening. On the other hand, after insertions or insertion attempts of the functional palp, both palps were involved in bouts of palpal moistening. In pairings of normal males with females having sealed copulatory pores, many of the groups of insertion at- tempts culminated in a bout of palpal moistening, which always began with the palp used in the attempts. Males having both palps fixed dorsad and males lacking both palps performed behavior associated with moistening of the unavailable palps. After adopting a medial position above the female's carapace and tilting his body caudad, such a male alternately spread and closed his chelicerae for a period of time in a manner similar to that
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Table 11.
Percentages of male Lycosa rabida performing various behaviors during copulation.
(This summary was obtained by appropriate re-groupings of the data presented in Table I.)
% %
'j% Tying % Pseudo-
Courtship Disorientation down insertion
Both 8 palps
unavailable 80.0 66.7 46.7 -
One $ palp
unavailable 53.3 13.3 20.0 13.3
One or both 9
pores unavailable 30.0 0.0 0.0 80.0
å and 9
untreated 0.0 0.0 0.0 0.0
seen during normal moistening. Likewise, those males in which only one palp was fixed dorsad alternated moistening of the available palp with periods in which the cheliceral movement continued without the available palp being drawn between the chelicerae. Males unable to insert one palp due to unilateral modification of themselves or their partners usually shifted over to that unavailable side after a successful insertion. After a bout of attempted insertions and palpal moistening or a period of inactivity on the unavailable side, the male shifted back to the functional side. In other words, throughout most of the copulation such males maintained a pattern of right-left alternation and did not remain on one side or the other for an extensive period of time. Towards the end of the mating, some of the males paired with females having one pore sealed did tend to perform an increased number of insertion attempts on the sealed side. Another irregularity in the right-left pattern which occurred occasionally in males of various experimental pairings was the attempted use of the "wrong" palp while on one side of the fe- male. For example, while on the female's right side, the male would press his left palp against the base of the female's right leg IV and achieve a partial (or, rarely, complete) hematodochal expansion. Females paired with males lacking both palps or males having both palps fixed dorsad showed appropriate abdominal swiveling when the male shifted from one side to the other. Abdominal swiveling was also elicited experimentally in females that had remained in the so-called cataleptic state after the male's dismount, as well as in a few females which had already resumed an active state, e.g. a de- fensive posture. In the latter instances, the females returned to the passive copulatory condition when I pressed down on their posterior carapace with a probe. Abdominal swiveling was subsequently elicited in the below-described manner. It was also possible to elicit abdorn-
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[September
Fig. 5.
Fernah Lyrosa rabida being tested after the male's dismount. She has responded to tactile stimulation of the left posterior region of her carapace by swiveling her abdomen so as to raise the left side dorsad. (Note that the mediay dark band on her abdomen is in line with the ri~ht dark band on her carapace.)
ilia1 swiveling in a few females that had initiated catalepsis prior to the male's mount in apparent rtspome to the mde's courtship dip play alone. In a11 of these cases, tactile stimulation of various regions of the female's cephalothmax arid abdomen with an artist bmh or a probe reveded that maximal abdominal swiveling was elicited by
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