Mammalian synthetic transcriptional regulation

Recent advances in the engineering of DNA binding domains have opened up the possibility of greatly expanding orthogonal sets of transcriptional regulators.

While bacterial promoters are relatively easy to manipulate, their mammalian counterparts can range from a few hundred base pairs to tens of thousands of base pairs in size and contain numerous binding sites for intricately interacting regulatory factors.

This project aims to construct an orthogonal set of mammalian transcriptional repression promoter-transcription factor pairs using modular DNA binding domains.

People: Zhen Xie; Yinqing Li