Foundation Projects


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Assembly and delivery of genetic circuits

Fundamental to mammalian synthetic biology is the ability to reliably construct and deliver synthetic gene circuit into the cell and to have the circuit function over time. Current methods include co-transfection of multiple plasmid and transfection of single plasmids with …


Mammalian synthetic transcriptional regulation

Recent advances in the engineering of DNA binding domains have opened up the possibility of greatly expanding orthogonal sets of transcriptional regulators. While bacterial promoters are relatively easy to manipulate, their mammalian counterparts can range from a few hundred base …


Rapid assembly and delivery of large scale genetic circuits into mammalian cells

This project aims to develop a framework to rapidly and reliably assemble and deliver large scale genetic circuits site-specifically into mammalian cells. The methods we’re developing allow choosing parts from a library of known (sequenced, characterized) mammalian parts and assembling …


Regulatory networks utilizing protein-protein interactions

In nature, cells respond to diverse stimuli over multiple length and time scales, but most synthetic gene circuits thus far have been limited to transcriptional regulation. We develop tools for Synthetic Biology to construct genetic circuits that encode synthetic protein …


Retroactivity

A central concept in synthetic biology is the construction of reusable, well-characterized modules. Modularity simplifies circuit design by allowing engineers to decouple systems into separate modules and construct and test modules individually. Instead of designing genetic circuits from scratch with …


Synthetic ecological system for pattern formation

This project aims at programming yeast cell life and death, and thereby study pattern formation, by integrating a synthetic genetic circuit into yeast cellular machinery. This engineering approach can be applied to cell density control in biotechnology industry, pattern formation …