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Create EcoRI to HindIII adapters

 

Materials:

tabular1268

Methods:

  1. EcoRI has the following matching sequence:

    tabular1218

  2. HindIII has the following matching sequence:

    tabular1239

  3. synthesize the following sequence: tex2html_wrap_inline3933
  4. synthesize the following sequence: tex2html_wrap_inline3935
  5. anneal the two sequences to get the following double-stranded DNA with sticky-ends:

    tex2html_wrap_inline3937
    tex2html_wrap_inline3939


Ron Weiss
Wed Feb 10 15:48:08 EST 1999